|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32103||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Growth instructionsBetter growth in JM109 (Primeplus cells)
Copy numberLow Copy
Gene/Insert nameTransformation/transcription domain-associated protein
SpeciesH. sapiens (human)
Insert Size (bp)16828
Entrez GeneTRRAP (a.k.a. DEDDFA, PAF350/400, PAF400, STAF40, TR-AP, Tra1)
- Promoter CMV
/ Fusion Protein
- Flag 1x (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site not1 (not destroyed)
- 3′ cloning site cla1 (not destroyed)
- 5′ sequencing primer CAG ATC GCC TGG AGA CGC C (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byConstructed by Michael D. Cole Lab.
Terms and Licenses
- Not Available to Industry
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CbS-Flag-TRRAP was a gift from Michael Cole & Yardena Samuels (Addgene plasmid # 32103 ; http://n2t.net/addgene:32103 ; RRID:Addgene_32103)