pCDNA3.1-CIB1-Myc
(Plasmid #32505)

• Depositing Labs: John Groffen, Nora Heisterkamp
• Publication: Haataja et al J Biol Chem. 2002 Mar 8;277(10):8321-8. Epub 2001 Dec 27.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CIB1
• Alt name: CIB1 calcium and integrin binding 1
• Alt name: CIB
• Species: H. sapiens (human)
• Insert Size (bp): 900
• GenBank ID: NM_006384.3
• Entrez Gene: CIB1 (a.k.a. CIB, CIBP, KIP1, PRKDCIP, SIP2-28)
• Tag / Fusion Protein:
  • Myc (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry