Dynamin I-K44A-non-tagged
(Plasmid #32695)

• Depositing Lab: Robert Lefkowitz
• Publication: Ahn et al J Biol Chem. 2002 Jul 19;277(29):26642-51. Epub 2002 May 13.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Dynamin I
• Species: R. norvegicus (rat)
• Insert Size (bp): 2000
• Mutation: K44A
• Entrez Gene: Dnm1 (a.k.a. Dnm)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-Fwd

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Sandra Schmid, Scripps Research Institute

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry