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pCS2 GFP-GSK3mut-MAPK
(Plasmid #29690)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 29690 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
  • Alt name
    GFP fused to mutated 3x GSK3 phosphorylation sites
  • Insert Size (bp)
    121
  • Mutation
    recognition sites only, and E3 ligase site, GSK3 sites mutated
  • Promoter SP6
  • Tags / Fusion Proteins
    • Flag-EGFP (N terminal on backbone)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This artificial GFP-GSK3mut-MAPK construct was generated by adding to the C terminus of GFP synthetic oligonucleotides encoding for AAAAAAAAAAAAAPASPAAAAAAAAPPAYDYKDDDDK. See Figure 6B of the associated publication for more details.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 GFP-GSK3mut-MAPK was a gift from Edward De Robertis (Addgene plasmid # 29690 ; http://n2t.net/addgene:29690 ; RRID:Addgene_29690)
  • For your References section:

    Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes. Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, Gumper I, Sabatini DD, De Robertis EM. Cell. 2010 Dec 23. 143(7):1136-48. 10.1016/j.cell.2010.11.034 PubMed 21183076