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Depositing Lab
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Depositing OrganizationYale University
Located in United States of America -
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 32782 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 32782-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 32782-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-N
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Backbone manufacturerClontech
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Modifications to backboneCMV promoter was replaced with 2500bp Tyrosinase promoter from mouse
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTyrosinase
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SpeciesH. sapiens (human)
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Entrez GeneTYR (a.k.a. ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3)
- Promoter Tyrosinase
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer EGFP_N and hTYR-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CMV promoter in pEGFP-N vector was replaced with 2500bp of tyrosinase endogenous promoter from mouse.
Please note that while the tyrosinase promoter is mouse, the sequence of the tyrosinase insert in this plasmid is human.
DNA (Catalog # 32782-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 32782-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ptyrEGFP-TYR was a gift from Ruth Halaban (Addgene plasmid # 32782 ; http://n2t.net/addgene:32782 ; RRID:Addgene_32782) -
For your References section:
Endoplasmic reticulum retention is a common defect associated with tyrosinase-negative albinism. Halaban R, Svedine S, Cheng E, Smicun Y, Aron R, Hebert DN. Proc Natl Acad Sci U S A. 2000 May 23;97(11):5889-94. 10.1073/pnas.97.11.5889 PubMed 10823941