Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32900||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3176
Vector typeMammalian Expression, RNAi
Growth in Bacteria
Copy numberHigh Copy
gRNA/shRNA sequence5’- GTAGCTCTCTGGTCACTCC -3’
SpeciesR. norvegicus (rat)
- Promoter H1-RNA
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
Can be used in conjunction with pcDNA3-rMEF2D (Addgene #32902) which is mutated so as to not be targeted by this shRNA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Mef2D-shRNA was a gift from Michael Greenberg (Addgene plasmid # 32900 ; http://n2t.net/addgene:32900 ; RRID:Addgene_32900)
For your References section:Activity-dependent regulation of MEF2 transcription factors suppresses excitatory synapse number. Flavell SW, Cowan CW, Kim TK, Greer PL, Lin Y, Paradis S, Griffith EC, Hu LS, Chen C, Greenberg ME. Science. 2006 Feb 17;311(5763):1008-12. 10.1126/science.1122511 PubMed 16484497