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pCVA-amiR-PDS
(Plasmid #32911)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32911 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAMBIA1301
  • Backbone size w/o insert (bp) 12000
  • Modifications to backbone
    amiRNA-PDS inserted
  • Vector type
    Plant Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    amiR-PDS (Nb)
  • Species
    N. benthamiana
  • Insert Size (bp)
    490

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The originally clone is received form Dr.Dominique Robertson (NC state University, USA)
  • Terms and Licenses

Depositor Comments

ami-R PDS sequence: 5'-TCAACATAGACTGATTGGGGC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCVA-amiR-PDS was a gift from Yule Liu (Addgene plasmid # 32911 ; http://n2t.net/addgene:32911 ; RRID:Addgene_32911)
  • For your References section:

    Virus-based microRNA expression for gene functional analysis in plants. Tang Y, Wang F, Zhao J, Xie K, Hong Y, Liu Y. Plant Physiol. 2010 Jun;153(2):632-41. Epub 2010 Apr 13. 10.1104/pp.110.155796 PubMed 20388670
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