Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32912||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5428
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
- Promoter CMV
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Article Citing this Plasmid
This is the acetylation-mimicking mutant.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:EGFP-Tubulin.K40Q was a gift from Tso-Pang Yao (Addgene plasmid # 32912 ; http://n2t.net/addgene:32912 ; RRID:Addgene_32912)
For your References section:The microtubule-associated histone deacetylase 6 (HDAC6) regulates epidermal growth factor receptor (EGFR) endocytic trafficking and degradation. Gao YS, Hubbert CC, Yao TP. J Biol Chem. 2010 Apr 9. 285(15):11219-26. 10.1074/jbc.M109.042754 PubMed 20133936