pcDNA6-rbN1 S77A
(Plasmid #32945)

• Depositing Lab: Edward Weinman
• Publication: Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297(6):F1560-5. Epub 2009 Sep 30.

Backbone

• Vector backbone: pcDNA6/HisC
• Backbone manufacturer: Invitrogene
• Backbone size w/o insert (bp): 5200
• Modifications to backbone: none
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SLc9a3r1
• Alt name: NHERF
• Species: rabbit
• Insert Size (bp): 1200
• Mutation: Change Serine77 to Alanine
• GenBank ID: u19815
• Entrez Gene: SLC9A3R1
• Promoter: CMV
• Tags / Fusion Proteins:
  • his (N terminal on backbone)
  • T7 tag (N terminal on insert)
  • partial FLAG (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: t7
• 3′ sequencing primer: bgh

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

NHERF-1 was cut from peGFP-rbNHERF-1 (ID#31636) constructs.Part of the peGFP vector is present at the 3' end. Serine77 was mutated to Alanine