Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||33351||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2800
Vector typeMammalian Expression, Mouse Targeting
Growth in Bacteria
Gene/Insert nameHsp68 promoter
SpeciesM. musculus (mouse)
Insert Size (bp)4500
Entrez GeneHspa1a (a.k.a. Hsp, Hsp7, Hsp70, Hsp70-3, Hsp70.3, Hsp72, hsp68, hsp70A1)
- Promoter Hsp68 (Hspa1a)
/ Fusion Protein
- LacZ (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer none
- 3′ sequencing primer LacZ-R (Common Sequencing Primers)
For more information about this plasmid see attached information sheet.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSfi-HspLacZ was a gift from David Kingsley & Doug Mortlock (Addgene plasmid # 33351 ; http://n2t.net/addgene:33351 ; RRID:Addgene_33351)
For your References section:A general approach for identifying distant regulatory elements applied to the Gdf6 gene. Mortlock DP, Guenther C, Kingsley DM. Genome Res. 2003 Sep;13(9):2069-81. Epub 2003 Aug 12. 10.1101/gr.1306003 PubMed 12915490
Map uploaded by the depositor.