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pBG100-TCPTP
(Plasmid #33365)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 33365 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBG100
  • Backbone manufacturer
    Vanberbilt structural biology core plasmid
  • Modifications to backbone
    pET27 derivative
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TCPTP-45
  • Species
    H. sapiens (human)
  • Entrez Gene
    PTPN2 (a.k.a. PTN2, PTPT, TC-PTP, TCELLPTP, TCPTP)
  • Promoter T7lac
  • Tag / Fusion Protein
    • 6xHIS - cleavable (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T7 forward
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBG100-TCPTP was a gift from Ambra Pozzi (Addgene plasmid # 33365 ; http://n2t.net/addgene:33365 ; RRID:Addgene_33365)
  • For your References section:

    Integrin {alpha}1{beta}1 promotes caveolin-1 dephosphorylation by activating T cell protein-tyrosine phosphatase. Borza CM, Chen X, Mathew S, Mont S, Sanders CR, Zent R, Pozzi A. J Biol Chem. 2010 Dec 17;285(51):40114-24. Epub 2010 Oct 12. 10.1074/jbc.M110.156729 PubMed 20940300