PTP1B human
(Plasmid #102719)

• Purpose: human PTP1B catalytic domain (residues 1-301) in RP1B vector
• Depositing Lab: Wolfgang Peti
• Publication: Choy et al Mol Cell. 2017 Feb 16;65(4):644-658.e5. doi: 10.1016/j.molcel.2017.01.014.

Backbone

• Vector backbone: RP1B
• Backbone manufacturer: laboratory designed; pETRP1 modified
• Backbone size w/o insert (bp): 5336
• Total vector size (bp): 6239
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Protein Tyrosine Phosphatase 1B (PTP1B)
• Species: H. sapiens (human)
• Insert Size (bp): 903
• Entrez Gene: PTPN1 (a.k.a. PTP1B)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-terminator

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry