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PTP1B human
(Plasmid #102719)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 102719 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RP1B
  • Backbone manufacturer
    laboratory designed; pETRP1 modified
  • Backbone size w/o insert (bp) 5336
  • Total vector size (bp) 6239
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Protein Tyrosine Phosphatase 1B (PTP1B)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    903
  • Entrez Gene
    PTPN1 (a.k.a. PTP1B)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-terminator
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PTP1B human was a gift from Wolfgang Peti (Addgene plasmid # 102719 ; http://n2t.net/addgene:102719 ; RRID:Addgene_102719)
  • For your References section:

    Conformational Rigidity and Protein Dynamics at Distinct Timescales Regulate PTP1B Activity and Allostery. Choy MS, Li Y, Machado LE, Kunze MB, Connors CR, Wei X, Lindorff-Larsen K, Page R, Peti W. Mol Cell. 2017 Feb 16;65(4):644-658.e5. doi: 10.1016/j.molcel.2017.01.014. 10.1016/j.molcel.2017.01.014 PubMed 28212750