Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #34582)


Item Catalog # Description Quantity Price (USD)
Plasmid 34582 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 5400
  • Modifications to backbone
    YFP open reading frame was amplified using specific primers and cloned into the p3xFlag-CMV-7.1 vector (Sigma) using BamHI and EcoRI restriction sites. Leaves HindIII, NotI, EagI and EcoRI sites between 3xHA and EYFP.
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on backbone)
    • EYFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3xFLAG-EYFP was a gift from Celeste Nelson (Addgene plasmid # 34582 ; ; RRID:Addgene_34582)
  • For your References section:

    Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. Lee K, Gjorevski N, Boghaert E, Radisky DC, Nelson CM. EMBO J. 2011 May 24;30(13):2662-74. doi: 10.1038/emboj.2011.159. 10.1038/emboj.2011.159 PubMed 21610693