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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 34587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 6299
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Modifications to backboneEGFP replaced with PAmKate
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePAmKate
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MutationF84W/S148N/S165G compared to mKate (but numbering relative to EGFP)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPAmKate-N1 was a gift from Vladislav Verkhusha (Addgene plasmid # 34587 ; http://n2t.net/addgene:34587 ; RRID:Addgene_34587) -
For your References section:
Superresolution imaging of multiple fluorescent proteins with highly overlapping emission spectra in living cells. Gunewardene MS, Subach FV, Gould TJ, Penoncello GP, Gudheti MV, Verkhusha VV, Hess ST. Biophys J. 2011 Sep 21;101(6):1522-8. Epub 2011 Sep 20. 10.1016/j.bpj.2011.07.049 PubMed 21943434
