mg180
(Plasmid #34660)

• Depositing Lab: Miriam Goodman
• Depositing Organization: Stanford University
  Located in United States of America
• Publication: Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.
• Citations: 1 Article

Backbone

• Vector backbone: pBluescript KS(+)
• Backbone size w/o insert (bp): 2958
• Vector type: Xenopus oocyte expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Top 10 F' E. coli
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: slo-1(A2;B0;C1)
• Alt name: BK channel
• Alt name: slo-1a
• Alt name: Lablife ID:L1089.P200
• Species: C. elegans (nematode)
• Insert Size (bp): 3760
• GenBank ID: NM_001029090
• Entrez Gene: slo-1 (a.k.a. CELE_Y51A2D.19)
• Tags / Fusion Proteins:
  • Xenopus beta-globulin 5' UTR (N terminal on insert)
  • Xenopus beta-globulin 3' UTR (C terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The backbone plasmid containing insert slo-1(A2;B0;C1) was obtained from Zhao-Wen Wang (University of Connecticut)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The backbone plasmid containing insert slo-1(A2;B0;C1) was first published in: Wang, Z.W., Saifee, O., Nonet, M.L., and Salkoff, L. (2001). SLO-1 potassium channels control quantal content of neurotransmitter release at the C. elegans neuromuscular junction. Neuron 32, 867-881.

DNA (Catalog # 34660-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 34660-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.