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pEYFP-Synectin
(Plasmid #35791)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 35791 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-C
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Synectin
  • Alt name
    Syn
  • Alt name
    Gipc1
  • Species
    M. musculus (mouse)
  • Mutation
    K111R
  • Entrez Gene
    Gipc1 (a.k.a. GIPC, Glut1CIP, Rgs19ip1, Semcap1, TIP-2, TaxIP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This construct contains a K111R mutation when compared to GenBank ID NP_061241.1 This mutation is most likely a sequence conflict, one of several (see Uniprot entry Q9Z0G0). The plasmid is exactly as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEYFP-Synectin was a gift from Arie Horowitz (Addgene plasmid # 35791 ; http://n2t.net/addgene:35791 ; RRID:Addgene_35791)
  • For your References section:

    Binding of internalized receptors to the PDZ domain of GIPC/synectin recruits myosin VI to endocytic vesicles. Naccache SN, Hasson T, Horowitz A. Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12735-40. Epub 2006 Aug 14. 10.1073/pnas.0605317103 PubMed 16908842