pEYFP-Synectin
(Plasmid #35791)

• Depositing Lab: Arie Horowitz
• Publication: Naccache et al Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12735-40. Epub 2006 Aug 14.

Backbone

• Vector backbone: pEYFP-C
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Synectin
• Alt name: Syn
• Alt name: Gipc1
• Species: M. musculus (mouse)
• Mutation: K111R
• Entrez Gene: Gipc1 (a.k.a. GIPC, Glut1CIP, Rgs19ip1, Semcap1, TIP-2, TaxIP2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct contains a K111R mutation when compared to GenBank ID NP_061241.1 This mutation is most likely a sequence conflict, one of several (see Uniprot entry Q9Z0G0). The plasmid is exactly as described in the associated publication.