Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||35943||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5250
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneGipc1 (a.k.a. GIPC, Glut1CIP, Rgs19ip1, Semcap1, TIP-2, TaxIP2)
- Promoter CMV
/ Fusion Proteins
- Xpress (N terminal on backbone)
- 6xHIS (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NA (unknown if destroyed)
- 3′ cloning site NA (unknown if destroyed)
- 5′ sequencing primer CMV fwd (Common Sequencing Primers)
This construct contains a K111R mutation when compared to GenBank ID NP_061241.1 This mutation is most likely a sequence conflict, one of several (see Uniprot entry Q9Z0G0). The plasmid is exactly as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA4/HisMax-Synectin was a gift from Arie Horowitz (Addgene plasmid # 35943 ; http://n2t.net/addgene:35943 ; RRID:Addgene_35943)
For your References section:Binding of internalized receptors to the PDZ domain of GIPC/synectin recruits myosin VI to endocytic vesicles. Naccache SN, Hasson T, Horowitz A. Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12735-40. Epub 2006 Aug 14. 10.1073/pnas.0605317103 PubMed 16908842