aq-CFP
(Plasmid #36073)

• Depositing Lab: Narasimhan Gautam
• Publication: Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G protein subunit alpha q
• Alt name: Gnaq
• Species: M. musculus (mouse)
• Insert Size (bp): 1077
• Mutation: CFP inserted between amino acid 124 and 125
• GenBank ID: NM_008139
• Entrez Gene: Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, Dsk1, Dsk10, Galphaq, Gq, GqI)
• Promoter: CMV
• Tag / Fusion Protein:
  • CFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: BGH reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry