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(Plasmid #36295)


Item Catalog # Description Quantity Price (USD)
Plasmid 36295 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 5678
  • Vector type
    Mammalian Expression
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • BT-tag (peptide sequence from P.freudenreichii transcarboxylase, that is biotinylated in vivo+TEV-site (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer ctcaagcctcagacagtgg
  • 3′ sequencing primer GAGGGGTGCAAGGACCCAG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB-BT was a gift from Ezra Burstein (Addgene plasmid # 36295 ; ; RRID:Addgene_36295)
  • For your References section:

    A bimolecular affinity purification method under denaturing conditions for rapid isolation of a ubiquitinated protein for mass spectrometry analysis. Maine GN, Li H, Zaidi IW, Basrur V, Elenitoba-Johnson KS, Burstein E. Nat Protoc. 2010 Aug;5(8):1447-59. Epub 2010 Jul 22. 10.1038/nprot.2010.109 PubMed 20671728