Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36353||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerSteve Elledge
- Backbone size w/o insert (bp) 6699
Vector typeMammalian Expression, Retroviral, RNAi
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneBMI1 (a.k.a. FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pSMP-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSMP-Bmi1_2 was a gift from George Daley (Addgene plasmid # 36353 ; http://n2t.net/addgene:36353 ; RRID:Addgene_36353)
For your References section:Chromatin-modifying enzymes as modulators of reprogramming. Onder TT, Kara N, Cherry A, Sinha AU, Zhu N, Bernt KM, Cahan P, Marcarci BO, Unternaehrer J, Gupta PB, Lander ES, Armstrong SA, Daley GQ. Nature. 2012 Mar 4;483(7391):598-602. doi: 10.1038/nature10953. 10.1038/nature10953 PubMed 22388813
Map uploaded by the depositor.