pGEX-2T Crkl SH3SH3
(Plasmid #36403)

• Depositing Lab: Nora Heisterkamp
• Publication: de Jong et al J Biol Chem. 1995 Sep 15;270(37):21468-71.

Backbone

• Vector backbone: pGEX-2T
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Crkl tandem SH3 domains
• Species: H. sapiens (human)
• Insert Size (bp): 630
• Entrez Gene: CRKL
• Tag / Fusion Protein:
  • GST (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sma I (destroyed during cloning)
• 3′ cloning site: Sma I (destroyed during cloning)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct includes the N- and C-terminal SH3 domains of Crkl, including residues 115-303. It is a 630 bp BsmI fragment (blunt) cloned into the SmaI site of pGEX-2T. Constructed by johanna ten Hoeve. Additional reference PMID 9053848.