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pGEX-2T Crkl SH3SH3
(Plasmid #36403)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 36403 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Crkl tandem SH3 domains
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sma I (destroyed during cloning)
  • 3′ cloning site Sma I (destroyed during cloning)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct includes the N- and C-terminal SH3 domains of Crkl, including residues 115-303. It is a 630 bp BsmI fragment (blunt) cloned into the SmaI site of pGEX-2T. Constructed by johanna ten Hoeve. Additional reference PMID 9053848.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2T Crkl SH3SH3 was a gift from Nora Heisterkamp (Addgene plasmid # 36403 ; ; RRID:Addgene_36403)
  • For your References section:

    Crkl is complexed with tyrosine-phosphorylated Cbl in Ph-positive leukemia. de Jong R, ten Hoeve J, Heisterkamp N, Groffen J. J Biol Chem. 1995 Sep 15;270(37):21468-71. 10.1074/jbc.270.37.21468 PubMed 7545163