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Bcr GAP domain R1090A/pGEX 3X
(Plasmid #36406)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 36406 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pGEX 3X
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Bcr GAP domain encoding residues 1004-1271 with R1090A point mutation
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Entrez Gene
    BCR (a.k.a. ALL, BCR1, CML, D22S11, D22S662, PHL)
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX-3X
  • 3′ sequencing primer pGEX-3X
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We constructed this in a 3-way ligation. The 5' end was a 0.16 kb BamHI-BstEII fragment from plasmid Bcr GAP domain in pGEX-3X, which provided the 5' BamHI site from pGEX-3X. The 3' end was a 1.2 kb BstEII-EcoRI fragment carrying the R1090A mutation. This was cloned into pGEX-3X digested with BamHI-EcoRI. The construct encodes the GAP-dead domain of Bcr which is inactive against GTP-bound Rac. It includes the natural stop codon and 3' untranslated sequences of the cDNA. Constructed by Nora Heisterkamp. The Rosetta2 strain may improve yield of the fusion protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Bcr GAP domain R1090A/pGEX 3X was a gift from Nora Heisterkamp (Addgene plasmid # 36406 ; ; RRID:Addgene_36406)
  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687