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Hox A13a
(Plasmid #36463)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 36463 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEM-T
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Riboprobe Synthesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hoxA13a
  • Alt name
    homeobox A13a
  • Species
    D. rerio (zebrafish)
  • Mutation
    contains aa102-290; M107T
  • GenBank ID
    570239
  • Entrez Gene
    hoxa13a (a.k.a. hoxa13)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site None (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's sequencing results indicate the Hox A13a insert in this plasmid starts at amino acid 102, contains a M107T mutation and ends at aa290 when compared to GenBank entry NP_001078963.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hox A13a was a gift from Robert Ho (Addgene plasmid # 36463 ; http://n2t.net/addgene:36463 ; RRID:Addgene_36463)
  • For your References section:

    Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: implications for the evolution of vertebrate paired appendages. Ahn D, Ho RK. Dev Biol. 2008 Oct 1;322(1):220-33. Epub 2008 Jul 3. 10.1016/j.ydbio.2008.06.032 PubMed 18638469