Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||36463||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typeRiboprobe Synthesis
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt namehomeobox A13a
SpeciesD. rerio (zebrafish)
Mutationcontains aa102-290; M107T
Entrez Genehoxa13a (a.k.a. hoxa13)
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site None (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequencing results indicate the Hox A13a insert in this plasmid starts at amino acid 102, contains a M107T mutation and ends at aa290 when compared to GenBank entry NP_001078963.1. The depositing laboratory believes these are allelic differences common with outbred strains and this is the exact plasmid they used in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Hox A13a was a gift from Robert Ho (Addgene plasmid # 36463 ; http://n2t.net/addgene:36463 ; RRID:Addgene_36463)
For your References section:Tri-phasic expression of posterior Hox genes during development of pectoral fins in zebrafish: implications for the evolution of vertebrate paired appendages. Ahn D, Ho RK. Dev Biol. 2008 Oct 1;322(1):220-33. Epub 2008 Jul 3. 10.1016/j.ydbio.2008.06.032 PubMed 18638469