pClneoFLAG human RASSF4
(Plasmid #37020)

• Depositing Lab: Yutaka Hata
• Publication: Ikeda et al Sci Signal. 2009 Sep 29;2(90):ra59.

Backbone

• Vector backbone: pCl-neo (modified)
• Backbone manufacturer: Promega
• Modifications to backbone: pCI-neo vector was modified to include FLAG tag N-terminal to MCS.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: rassf4
• Species: H. sapiens (human)
• GenBank ID: BC032593
• Entrez Gene: RASSF4 (a.k.a. AD037)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cDNA encoding human RASSF4 (BC032593) was purchased from Open Biosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results indicate there are two EcoRI sites after FLAG