Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||37160||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)E. Coli VJS6737
Copy numberHigh Copy
Gene/Insert namercoM1 in pEXT20
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer pEXT20-F2 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This strain contains Roberts lab plasmid pUX2396 which contains an 831-bp EcoRI-HindIII fragment bearing Burkholderia xenovorans LB400 rcoM1 cloned into pEXT20. Suitable for expression of RcoM1
protein (untagged). Host strain is E. coli VJS6737.
For strain usage see attached pdf.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:UQ4802 was a gift from Gary Roberts (Addgene plasmid # 37160)
For your References section:RcoM: a new single-component transcriptional regulator of CO metabolism in bacteria. Kerby RL, Youn H, Roberts GP. J Bacteriol. 2008 May;190(9):3336-43. Epub 2008 Mar 7. 10.1128/JB.00033-08 PubMed 18326575