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pCP5b
(Plasmid #37185)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37185 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCP5
  • Backbone manufacturer
    Voytas lab (Addgene Plasmid #35397)
  • Backbone size (bp) 13853
  • Modifications to backbone
    Plasmid pCP5 yeast 2micron origin of replication and Kanamycin resistance gene replaced with pBR322 origin of replication and Ampicilin resistance cassette from pBluescript SK (to increase replication yield)
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Depositor Comments

pCP5b is a high copy derivative of pCP5 for higher DNA yield from bacteria.
Plasmid used to assay TALEN function in yeast. Clone target sequences into BglII and SpeI sites as described on article.
Grow yeast transformants in SD-Ura-Trp prior to assay to prevent spontaneous recombination between the lacZ fragments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCP5b was a gift from Adam Bogdanove (Addgene plasmid # 37185 ; http://n2t.net/addgene:37185 ; RRID:Addgene_37185)
  • For your References section:

    Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Cermak T, Doyle EL, Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. 2011 Apr 14. ():. 10.1093/nar/gkr218 PubMed 21493687