pFoxd3IRESGFPtv
(Plasmid #37266)

• Depositing Lab: Patricia Labosky
• Publication: Hanna et al Genes Dev. 2002 Oct 15;16(20):2650-61.

Backbone

• Vector backbone: pBS
• Backbone manufacturer: Stratgene
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 15000
• Modifications to backbone: The targeting vector was constructed using a 4.0 kilobase (kb) Sac1 fragment as the 5
  homology arm and a 1.5kb Xba1 fragment as the 3
  arm. A diptheria toxin (DTA) cassette was used to select against random integration, and positive selection was provided by the neo cassette, flanked by loxP sites for subsequent removal in culture. The Foxd3 targeted allele has the entire coding region deleted as well as  70 bp of the 3
  flanking DNA. A Gfp gene with an internal ribosomal entry site (Ires, for the Foxd3Ires-Gfp allele) was inserted as described in Hanna et al.
• Vector type: Mouse Targeting
• Selectable markers: Neomycin (select with G418) ; DTA

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Foxd3
• Alt name: Hfh2
• Alt name: Genesis
• Species: M. musculus (mouse)
• Insert Size (bp): 13000
• GenBank ID: 1347473
• Entrez Gene: Foxd3 (a.k.a. CWH3, Gen, Genesis, Hfh2)
• Tag / Fusion Protein:
  • IRES-GFP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry