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pRK5 Flag Grb10 wt long
(Plasmid #37481)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37481 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Grb10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1785
  • Mutation
    isoform a, 594aa
  • GenBank ID
    NM_005311.4
  • Entrez Gene
    GRB10 (a.k.a. GRB-IR, Grb-10, IRBP, MEG1, RSS)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5 Flag Grb10 wt long was a gift from David Sabatini (Addgene plasmid # 37481 ; http://n2t.net/addgene:37481 ; RRID:Addgene_37481)
  • For your References section:

    The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Hsu PP, Kang SA, Rameseder J, Zhang Y, Ottina KA, Lim D, Peterson TR, Choi Y, Gray NS, Yaffe MB, Marto JA, Sabatini DM. Science. 2011 Jun 10;332(6035):1317-22. 10.1126/science.1199498 PubMed 21659604