p6895 PHAGE-P CMVT N-HA-hE6AP I wt
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37601||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepHAGE-P CMVt N-HA GAW
- Backbone size w/o insert (bp) 7800
- Total vector size (bp) 10374
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameE6AP I wt
SpeciesH. sapiens (human)
Insert Size (bp)2556
Entrez GeneUBE3A (a.k.a. ANCR, AS, E6-AP, EPVE6AP, HPVE6A)
- Promoter CMV tet regulatable
/ Fusion Protein
- HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p6895 PHAGE-P CMVT N-HA-hE6AP I wt was a gift from Peter Howley (Addgene plasmid # 37601 ; http://n2t.net/addgene:37601 ; RRID:Addgene_37601)
For your References section:Identification and proteomic analysis of distinct UBE3A/E6AP protein complexes. Martinez-Noel G, Galligan JT, Sowa ME, Arndt V, Overton TM, Harper JW, Howley PM. Mol Cell Biol. 2012 May 29. 10.1128/MCB.00201-12 PubMed 22645313