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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 37627-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 37627-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL2 Basic
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 5598
- Total vector size (bp) 6918
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMDR1 promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1320
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Entrez GeneABCB1 (a.k.a. ABC20, CD243, CLCS, GP170, MDR1, P-GP, PGY1, p-170)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xma I (not destroyed)
- 3′ cloning site Nhe I (destroyed during cloning)
- 5′ sequencing primer GLprimer1
- 3′ sequencing primer GLprimer2
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid forms dimers (see diagnostic digest linked above).
DNA (Catalog # 37627-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 37627-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMDR1-1202 was a gift from Kathleen Scotto (Addgene plasmid # 37627 ; http://n2t.net/addgene:37627 ; RRID:Addgene_37627) -
For your References section:
Transcriptional activation of the MDR1 gene by UV irradiation. Role of NF-Y and Sp1. Hu Z, Jin S, Scotto KW. J Biol Chem. 2000 Jan 28;275(4):2979-85. 10.1074/jbc.275.4.2979 PubMed 10644769
