|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37674||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneEGFP removed from pEGFP-C1
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneSmo (a.k.a. E130215L21Rik, Smoh, bnb, smoothened)
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer CMV-Fwd
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
Addgene NGS results identified a D54N variant compared to the NCBI reference [EDL13766.1].
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGEN-mSmoA1 was a gift from Philip Beachy (Addgene plasmid # 37674 ; http://n2t.net/addgene:37674 ; RRID:Addgene_37674)
For your References section:Effects of oncogenic mutations in Smoothened and Patched can be reversed by cyclopamine. Taipale J, Chen JK, Cooper MK, Wang B, Mann RK, Milenkovic L, Scott MP, Beachy PA. Nature. 2000 Aug 31;406(6799):1005-9. 10.1038/35023008 PubMed 10984056