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pAcGP67A-rCL1-HormR-GAIN
(Plasmid #37847)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37847 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcGP67A
  • Backbone manufacturer
    BD Biosciences
  • Backbone size w/o insert (bp) 9761
  • Vector type
    Baculovirus Transfer Vectors

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CL1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1167
  • Mutation
    HormR and GAIN domains only (aa460–849
  • Entrez Gene
    Adgrl1 (a.k.a. CL1BA, Lphn1)
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • 8xHIS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer polyhedrin-fwd
  • 3′ sequencing primer polyhedrin-rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAcGP67A-rCL1-HormR-GAIN was a gift from Axel Brunger (Addgene plasmid # 37847 ; http://n2t.net/addgene:37847 ; RRID:Addgene_37847)
  • For your References section:

    A novel evolutionarily conserved domain of cell-adhesion GPCRs mediates autoproteolysis. Arac D, Boucard AA, Bolliger MF, Nguyen J, Soltis SM, Sudhof TC, Brunger AT. EMBO J. 2012 Feb 14;31(6):1364-78. doi: 10.1038/emboj.2012.26. 10.1038/emboj.2012.26 PubMed 22333914