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pPK700
(Plasmid #38146)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38146 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript KSII(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 14610
  • Vector type
    Worm Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    let-60p::ptc-3::gfp
  • Alt name
    let-60 promoter
  • Alt name
    ptc-3
  • Species
    C. elegans (nematode)
  • Entrez Gene
    ptc-3 (a.k.a. Y110A2AL.8)
  • Promoter let-60
  • Tag / Fusion Protein
    • EGFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13pUC-rev
  • 3′ sequencing primer M13pUC-fwd
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The pPK700 plasmid was derived by ligating the let-60 promoter region to ptc-3::gfp expression cassette, which was PCR amplified from pPK348 (Addgene plasmid 38145)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPK700 was a gift from Patricia Kuwabara (Addgene plasmid # 38146 ; http://n2t.net/addgene:38146 ; RRID:Addgene_38146)
  • For your References section:

    C. elegans patched-3 is an essential gene implicated in osmoregulation and requiring an intact permease transporter domain. Soloviev A, Gallagher J, Marnef A, Kuwabara PE. Dev Biol. 2011 Mar 15;351(2):242-53. Epub 2011 Jan 4. 10.1016/j.ydbio.2010.12.035 PubMed 21215260