|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38145||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript KSII(+)
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 16304
Vector typeWorm Expression
Growth in Bacteria
Growth Strain(s)XL1 Blue
SpeciesC. elegans (nematode)
Entrez Geneptc-3 (a.k.a. Y110A2AL.8)
- Promoter ptc-3
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13pUC-rev (Common Sequencing Primers)
Terms and Licenses
The pPK348 plasmid was derived by inserting the GFP cassette from pPD103.87 (Addgene plasmid 1524) flanked by XbaI ends into the unique SpeI site present in exon 8 of ptc-3 and carries the entire predicted ptc-3 locus comprised of a 6.9 kb genomic coding region fused in-frame to GFP and more than 5 kb of 5′ upstream sequence, including the putative promoter
Please note that Addgene's sequencing results identified several mismatches in the region of bp#722-824 when compared to the full plasmid sequence provided by the depositing laboratory. The depositor states that these mismatches are not a concern for the function of the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPK348 was a gift from Patricia Kuwabara (Addgene plasmid # 38145 ; http://n2t.net/addgene:38145 ; RRID:Addgene_38145)
For your References section:C. elegans patched-3 is an essential gene implicated in osmoregulation and requiring an intact permease transporter domain. Soloviev A, Gallagher J, Marnef A, Kuwabara PE. Dev Biol. 2011 Mar 15;351(2):242-53. Epub 2011 Jan 4. 10.1016/j.ydbio.2010.12.035 PubMed 21215260