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(Plasmid #38174)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 38174 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 7800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    ABR transcript variant 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    addition of a Src myristoylation sequence (see comments)
  • Entrez Gene
    ABR (a.k.a. MDB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Constructed by Young Jin Cho. See Supplemental Fig. S3B in PMID 17116687 for membrane location.
Mutagenesis done using Stratagene Quick Change kit. This is ABR transcript 2/isoform B. Abr in pAcG2T (AddGene plasmid id 38162; this parent plasmid may contain an E24G mutation present -gag to ggg- introduced by Taq polymerase at an earlier cloning step) was used to introduce the Src myristoylation sequence at the N-temrnial end of Abr. We inserted 6 amino acids into the original sequence (BamHI-) GGATCC ATG GAG GAG GAA GAG GAG GCG…to get (KpnI-) GGTACC ATG GGC AGC AGC AAG AGC AAG GAG GAG GAA GAG etc. The first amino acids now are M-G-S-S-K-S-K-
The plasmid was digested with KpnIxEcoRI and the 2.8 kb insert ligated into pcDNA3.1(+) digested with KpnIxEcoRI. The construct includes the natural stop codon and 3’UT sequences but the protein does not contain a tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    myr-Abr was a gift from Nora Heisterkamp (Addgene plasmid # 38174 ; ; RRID:Addgene_38174)
  • For your References section:

    Abr and Bcr, two homologous Rac GTPase-activating proteins, control multiple cellular functions of murine macrophages. Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N. Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20. 10.1128/MCB.00756-06 PubMed 17116687