pγsat
(Plasmid #39238)

• Depositing Lab: Niall Dillon
• Publication: Lundgren et al Cell. 2000 Nov 22;103(5):733-43.

Backbone

• Vector backbone: low copy pBluescript
• Backbone size w/o insert (bp): 3806
• Vector type: Cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl3
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 8 copies of the 234bp mouse γ-satellite repeat
• Alt name: Heterochromatin FISH Probe
• Alt name: pgammasat
• Species: M. musculus (mouse)
• Insert Size (bp): 1900

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: T3
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The four satellite repeats, which are duplicated in the construct, were generated by PCR from mouse DNA, so they represent a native mouse satellite array. The sequence is a consensus, however deviation from the consensus sequence ranging from 0.9 to 9.1% (mean deviation 3.9%) has been reported. The construct is mainly used as a FISH probe for visualising pericentromeric regions in mouse cells and this is unlikely to be affected by minor sequence variations. Another important point about this construct is that contains 8 direct repeats and the 234 bp satellite sequence also contains internal repeats. This will make it prone to rearrangements when it is grown up for isolation, which means that it will be difficult to completely guarantee its integrity.

For reference please see - Vissel, B. and Choo KH. (1989). Mouse major (gamma) satellite DNA is highly conserved and organised into extremely long tandem arrays: Implications for recombination between non-homologous chromosomes. Genomics 5, 407-414.