pSANG14-3F
(Plasmid #39265)

• Purpose: (Empty Backbone)
• Depositing Lab: John McCafferty
• Publication: Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

Backbone

• Vector backbone: pSANG10-3F
• Backbone manufacturer: Addgene plasmids #39264
• Backbone size (bp): 5438
• Modifications to backbone: The alkaline phosphatase encoding gene was inserted between the scFv and 6xHis tag in pSANG10-3F to create pSANG14-3F. The scFv encoding gene is sub-cloned at the NcoI/NotI site and the 5' HindIII site is available for insertion of peptide tags and fusion partners.
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • pelB leader (N terminal on insert)
  • Alkaline phosphatase (C terminal on insert)
  • 6xHis (C terminal on insert)
  • tri-FLAG (C terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Must be transformed into BL21(DE3) bacteria and grown at 30 degrees for antibody production.
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminal primer

Resource Information

• Supplemental Documents:
  • pSANG14-3F.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Bacterial expression of recombinant antibody fragments in the form of single chain Fvs (scFvs), dimerised through E. coli alkaline phosphatase