Sequences (2) — Accept Affinity Reagent Sequence Policy
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39345||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namescFv specific for Jagged (clone D5)
SpeciesH. sapiens (human)
Entrez GeneJAG1 (a.k.a. AGS, AGS1, AHD, AWS, CD339, CMT2HH, DCHE, HJ1, JAGL1)
/ Fusion Proteins
- 6xHis (C terminal on backbone)
- 3xFLAG (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH_rev_primer (Common Sequencing Primers)
Article Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:anti-Jagged_D5-pBIOCAM5 was a gift from John McCafferty (Addgene plasmid # 39345 ; http://n2t.net/addgene:39345 ; RRID:Addgene_39345)
For your References section:Application of phage display to high throughput antibody generation and characterization. Schofield DJ, Pope AR, Clementel V, Buckell J, Chapple SDj, Clarke KF, Conquer JS, Crofts AM, Crowther SR, Dyson MR, Flack G, Griffin GJ, Hooks Y, Howat WJ, Kolb-Kokocinski A, Kunze S, Martin CD, Maslen GL, Mitchell JN, O'Sullivan M, Perera RL, Roake W, Shadbolt SP, Vincent KJ, Warford A, Wilson WE, Xie J, Young JL, McCafferty J. Genome Biol. 2007;8(11):R254. 10.1186/gb-2007-8-11-r254 PubMed 18047641