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pRc/CMV HA TrkB-T1
(Plasmid #39980)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 39980 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pRC/CMV AC7 vector, a derivative of the pRC/CMV vector
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    NTrk2 variant 2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
    TrkB.T1 variant; endogenous signal sequence was replaced with BM40 and HA tag
  • GenBank ID
  • Entrez Gene
    Ntrk2 (a.k.a. RATTRKB1, TRKB1, Tkrb, trk-B, trkB)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • BM40 signal sequence (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The T1 isoform of trkB was cloned from a pCMV-5 trkB.T1 construct using HindIII and XbaI to fuse it to the HA-tagged N-terminus of trkB in the vector pB-KS followed by subcloning into pRC/CMV AC7 with NheI and NotI.

See Middlemas et al (Mol Cell Biol. 1991 Jan;11(1):143-53., ) to learn more about the TrkB.T1 variant.

Please note that the endogenous signal sequence of TrkB-T1 was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequences for GenBank ID M55292.1 or NM_001163168.1, the TrkB-T1 protein sequence starts at amino acid #32

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRc/CMV HA TrkB-T1 was a gift from Yves-Alain Barde (Addgene plasmid # 39980 ; ; RRID:Addgene_39980)
  • For your References section:

    Biochemical and functional interactions between the neurotrophin receptors trk and p75NTR. Bibel M, Hoppe E, Barde YA. EMBO J. 1999 Feb 1;18(3):616-22. 10.1093/emboj/18.3.616 PubMed 9927421