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CD40L-MIEG-hCD4 (hCD4-CD40L)
(Plasmid #40355)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40355 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MIEG-hCD4
  • Backbone manufacturer
    Addgene plasmid 40569
  • Modifications to backbone
    IRES EGFP of pMIEG replaced with human CD4 cDNA
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD40L
  • Alt name
    CD40-ligand
  • Alt name
    CD154
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Cd40lg (a.k.a. CD154, CD40-L, Cd40, Cd40l, HIGM, HIGM1, IGM, IMD, IMD3, Ly-6, Ly-62, Ly62, T-BA, T-BAM, TRAP, Tnfs, Tnfsf5, Tnlg8b, gp3, gp39)
  • Tag / Fusion Protein
    • IRES hCD4 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

The CD40-ligand (CD40L)-expressing retroviral vector was constructed by PCR amplification of cDNA from anti-CD3 activated mouse T cells with the following primers for CD40-ligand: sense 5'-CTTTCAGTCAGCATGATAGAAACA-3' and antisense
5'-TCAGAGTTTGAGTAAGCCAAAAGA-3'; these primers were used to amplify the complete coding region of mouse CD40 ligand. The CD40-ligand gene was then reamplified with primers containing XhoI and NotI sites and then cloned via XhoI and NotI sites into a retroviral vector expressing human CD4.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD40L-MIEG-hCD4 (hCD4-CD40L) was a gift from Alexander Dent (Addgene plasmid # 40355 ; http://n2t.net/addgene:40355 ; RRID:Addgene_40355)
  • For your References section:

    BCL6 cooperates with CD40 stimulation and loss of p53 function to rapidly transform primary B cells. Kusam S, Munugalavadla V, Sawant D, Dent A. Int J Cancer. 2009 Aug 15;125(4):977-81. 10.1002/ijc.24450 PubMed 19405121