Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40355||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid 40569
Modifications to backboneIRES EGFP of pMIEG replaced with human CD4 cDNA
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneCd40lg (a.k.a. CD154, CD40-L, Cd40, Cd40l, HIGM, HIGM1, IGM, IMD, IMD3, Ly-6, Ly-62, Ly62, T-BA, T-BAM, TRAP, Tnfs, Tnfsf5, Tnlg8b, gp3, gp39)
/ Fusion Protein
- IRES hCD4 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
The CD40-ligand (CD40L)-expressing retroviral vector was constructed by PCR amplification of cDNA from anti-CD3 activated mouse T cells with the following primers for CD40-ligand: sense 5'-CTTTCAGTCAGCATGATAGAAACA-3' and antisense
5'-TCAGAGTTTGAGTAAGCCAAAAGA-3'; these primers were used to amplify the complete coding region of mouse CD40 ligand. The CD40-ligand gene was then reamplified with primers containing XhoI and NotI sites and then cloned via XhoI and NotI sites into a retroviral vector expressing human CD4.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CD40L-MIEG-hCD4 (hCD4-CD40L) was a gift from Alexander Dent (Addgene plasmid # 40355 ; http://n2t.net/addgene:40355 ; RRID:Addgene_40355)
For your References section:BCL6 cooperates with CD40 stimulation and loss of p53 function to rapidly transform primary B cells. Kusam S, Munugalavadla V, Sawant D, Dent A. Int J Cancer. 2009 Aug 15;125(4):977-81. 10.1002/ijc.24450 PubMed 19405121