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pUHD.NFATc1nuc
(Plasmid #40616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40616 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 40616-D50 50 μg of DNA in Tris buffer $495
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DNA 40616-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUHD
  • Backbone size w/o insert (bp) 3100
  • Total vector size (bp) 5364
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NFATc1nuc
  • Alt name
    NF-ATC, NFAT2, NFATc
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2154
  • Mutation
    S172A S175A S176A S178A S179A S181A S184A S187A S188A S191A S194A S199A S203A S207A S211A S233A S237A S278A S282A S286A S290A
  • Entrez Gene
    NFATC1 (a.k.a. NF-ATC, NF-ATc1.2, NFAT2, NFATc)
  • Promoter CMV2
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site EcoR1 (destroyed during cloning)
  • 5′ sequencing primer pCEP-fwd primer
  • 3′ sequencing primer EBV_rev primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUHD.NFATc1nuc was a gift from Jerry Crabtree (Addgene plasmid # 40616 ; http://n2t.net/addgene:40616 ; RRID:Addgene_40616)

  • For your References section:

    Calcineurin/NFAT signaling in osteoblasts regulates bone mass. Winslow MM, Pan M, Starbuck M, Gallo EM, Deng L, Karsenty G, Crabtree GR. Dev Cell. 2006 Jun . 10(6):771-82. 10.1016/j.devcel.2006.04.006 PubMed 16740479
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