pLKO.1puro-shKIBRA-B
(Plasmid #40889)

• Depositing Lab: Paul Reynolds
• Publication: Moleirinho et al Oncogene. 2012 May 21. doi: 10.1038/onc.2012.196.

Backbone

• Vector backbone: pLKO.1 puro
• Backbone manufacturer: Addgene 8453
• Backbone size w/o insert (bp): 7032
• Total vector size (bp): 7079
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shKIBRA-B
• Alt name: WWC1
• gRNA/shRNA sequence: TGGTACAACCTTCTCAGCT
• Species: H. sapiens (human)
• Entrez Gene: WWC1 (a.k.a. HBEBP3, HBEBP36, KIBRA, MEMRYQTL, PPP1R168)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLKO.1 seq

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry