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pBABEpuro-ERBB2 A775_G776insV, G776C
(Plasmid #40979)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 40979 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBABE-puro
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 9000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERBB2
  • Alt name
    HER2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3768
  • Mutation
    A775_G776insV, G776C
  • GenBank ID
    NM_004448.2
  • Entrez Gene
    ERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer TTTGTACACCCTAAGCCTC
  • 3′ sequencing primer GCATACTTCTGCCTGCTG
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABEpuro-ERBB2 A775_G776insV, G776C was a gift from Matthew Meyerson (Addgene plasmid # 40979 ; http://n2t.net/addgene:40979 ; RRID:Addgene_40979)
  • For your References section:

    Functional analysis of receptor tyrosine kinase mutations in lung cancer identifies oncogenic extracellular domain mutations of ERBB2. Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, Meyerson M. Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug 20. 10.1073/pnas.1203201109 PubMed 22908275