Gcm2TEASY
(Plasmid #41031)

• Depositing Lab: Nancy Manley
• Publication: Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

Backbone

• Vector backbone: pGEM-T Easy
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3015
• Vector type: in situ probe

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gcm2 in situ probe
• Species: M. musculus (mouse)
• Mutation: contains nucleotides 584-1510 of GenBank accession number AF081556
• GenBank ID: AF081556
• Entrez Gene: Gcm2 (a.k.a. Gcm-rs1, Gcm1, Gcm1-rs2)
• Promoter: T7 sense; SP6 antisense

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SpeI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For Gcm2 in situ antisense probe, linearize with NcoI and transcribe with SP6