pBS Rootletin (Nigg pFL1(CW498))
(Plasmid #41168)

• Depositing Lab: Erich Nigg
• Publication: Bahe et al J Cell Biol. 2005 Oct 10;171(1):27-33. Epub 2005 Oct 3.

Backbone

• Vector backbone: pBluescript II SK(-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2961
• Total vector size (bp): 9000
• Modifications to backbone: FseI site inserted 5' to insert (in fwd cloning oligo)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rootletin
• Alt name: CROCC
• Alt name: ciliary rootlet coiled-coil, rootletin
• Species: H. sapiens (human)
• Insert Size (bp): 6000
• GenBank ID:
• Entrez Gene: CROCC (a.k.a. ROLT, TAX1BP2)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13_pUC_Fwd
• 3′ sequencing primer: M13_pUC_Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Partial cDNA clone KIAA0445 from Kazusa DNA Research Institute and IMAGE clone 6150861 from Deutsches Ressourcenzentrum fuer Genomforschung GmbH

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The partial cDNA clones KIAA0445 (Kazusa DNA Research Institute) and IMAGE clone 6150861 (Deutsches Ressourcenzentrum fuer Genomforschung GmbH) were fused, and the complete coding sequence for rootletin was cloned into pBluescript II SK-. Construct was confirmed by sequencing.