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pFA6a-kanMX6-PGAL1-GFP
(Plasmid #41614)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41614 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GAL1 promoter
  • Alt name
    GAL1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2807
  • Entrez Gene
    GAL1 (a.k.a. YBR020W)
  • Tags / Fusion Proteins
    • A. gossypii translation elongation factor 1a gene terminator (N terminal on insert)
    • KanR reverse orientation (N terminal on insert)
    • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
    • GFP(S65T) (C terminal on insert)
    • yeast ADH1 terminator (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T7_promoter
  • 3′ sequencing primer Sp6_primer
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-kanMX6-PGAL1-GFP was a gift from John Pringle (Addgene plasmid # 41614 ; http://n2t.net/addgene:41614 ; RRID:Addgene_41614)
  • For your References section:

    Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U PubMed 9717241