pCIneoMyc mouse Numb
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41712||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5472
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Mutationcontains amino acids 8-853
GenBank IDBC033459 AAH33459.1
Entrez GeneNumb (a.k.a. Nb, m-num)
- Promoter CMV
/ Fusion Protein
- Myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site SalI (unknown if destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer T3 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCIneoMyc mouse Numb was a gift from Yutaka Hata (Addgene plasmid # 41712 ; http://n2t.net/addgene:41712 ; RRID:Addgene_41712)
For your References section:Ligand-of-Numb protein X is an endocytic scaffold for junctional adhesion molecule 4. Kansaku A, Hirabayashi S, Mori H, Fujiwara N, Kawata A, Ikeda M, Rokukawa C, Kurihara H, Hata Y. Oncogene. 2006 Aug 24;25(37):5071-84. Epub 2006 Jul 10. 10.1038/sj.onc.1209468 PubMed 16832352