pCIneoMyc mouse Numb
(Plasmid #41712)

• Depositing Lab: Yutaka Hata
• Publication: Kansaku et al Oncogene. 2006 Aug 24;25(37):5071-84. Epub 2006 Jul 10.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5472
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Numb
• Species: M. musculus (mouse)
• Mutation: contains amino acids 8-853
• GenBank ID: BC033459 AAH33459.1
• Entrez Gene: Numb (a.k.a. Nb)
• Promoter: CMV
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: SalI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: T3

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry