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pCIneoMyc mouse Numb
(Plasmid #41712)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41712 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Numb
  • Species
    M. musculus (mouse)
  • Mutation
    contains amino acids 8-853
  • GenBank ID
    BC033459 AAH33459.1
  • Entrez Gene
    Numb (a.k.a. Nb)
  • Promoter CMV
  • Tag / Fusion Protein
    • Myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site SalI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCIneoMyc mouse Numb was a gift from Yutaka Hata (Addgene plasmid # 41712 ; http://n2t.net/addgene:41712 ; RRID:Addgene_41712)
  • For your References section:

    Ligand-of-Numb protein X is an endocytic scaffold for junctional adhesion molecule 4. Kansaku A, Hirabayashi S, Mori H, Fujiwara N, Kawata A, Ikeda M, Rokukawa C, Kurihara H, Hata Y. Oncogene. 2006 Aug 24;25(37):5071-84. Epub 2006 Jul 10. 10.1038/sj.onc.1209468 PubMed 16832352