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H9 F42A pAcGP67a
(Plasmid #41810)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41810 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAcGP67a
  • Backbone manufacturer
    BD
  • Backbone size w/o insert (bp) 9761
  • Total vector size (bp) 10000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H9 Super-2
  • Alt name
    Super-2
  • Alt name
    H9
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    429
  • Mutation
    Phenylalanine 42 to Alanine
  • Promoter Polyhedron
  • Tag / Fusion Protein
    • 8x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer PolyhedronFor
  • 3′ sequencing primer PolyhedronRev
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

H9 has the following mutations compared to wildtype IL-2: L80F, R81F, L85V, I86V, I92F

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H9 F42A pAcGP67a was a gift from Chris Garcia (Addgene plasmid # 41810 ; http://n2t.net/addgene:41810 ; RRID:Addgene_41810)
  • For your References section:

    Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'. Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC. Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975. 10.1038/nature10975 PubMed 22446627