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(Plasmid #87163)


Item Catalog # Description Quantity Price (USD)
Plasmid 87163 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5689
  • Total vector size (bp) 6454
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Promoter EF-1 alpha
  • Tags / Fusion Proteins
    • Bip signal peptide (Homo sapiens) (N terminal on insert)
    • mTagBFP-KDEL (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer pEF-forward tctctccacaggtgtccact
  • 3′ sequencing primer pEF-rev acaccggccttattccaagc
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    mTagBFP-KDEL from addgene plasmid #49150 (BFP-KDEL)
  • Article Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This vector was generated by Simon Pfisterer (Elina Ikonen Lab).

The BFP-KDEL derived from addgene plasmid #49150 was transferred to pEFIRES-P vector to facilitate the selection of stable mammalian cell lines.

Benefits of using pEFIRES-P vector for stable overexpression in mammalian cells were described by Stephen Hobbs et al. (Biochem Biophys Res Commun. 1998 Nov 18;252(2):368-72.).

pEFIRES-P was a gift from Dr. Olli Ritvos (University of Helsinki).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEFIRES-P-mTagBFP-KDEL was a gift from Elina Ikonen (Addgene plasmid # 87163 ; ; RRID:Addgene_87163)
  • For your References section:

    Seipin regulates ER-lipid droplet contacts and cargo delivery. Salo VT, Belevich I, Li S, Karhinen L, Vihinen H, Vigouroux C, Magre J, Thiele C, Holtta-Vuori M, Jokitalo E, Ikonen E. EMBO J. 2016 Dec 15;35(24):2699-2716. Epub 2016 Nov 22. 10.15252/embj.201695170 PubMed 27879284