pKT127-SEP
(Plasmid
#41899)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41899 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKT127
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Backbone manufacturerKurt S. Thorn
- Backbone size (bp) 4894
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Modifications to backboneReplaced yeGFP with superecliptic GFP (SEP)
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Vector typeYeast Expression
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Selectable markersG418
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Tag
/ Fusion Protein
- superecliptic GFP (SEP) (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pKT127-SEP, used for creating in-frame C-terminal chromosomal fusions of a gene of interest with SEP, was created by removing yeGFP from pKT127 (http://web.uni-frankfurt.de/fb15/mikro/euroscarf/data/pKT127.html) by restriction digestion with PacI/AscI and replacing it with PacI/AscI-digested SEP generated by PCR from template plasmid pGM87
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKT127-SEP was a gift from Dan Gottschling (Addgene plasmid # 41899 ; http://n2t.net/addgene:41899 ; RRID:Addgene_41899) -
For your References section:
An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Hughes AL, Gottschling DE. Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21. 10.1038/nature11654 PubMed 23172144
Map uploaded by the depositor.
